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Antibiotic resistance
Wound infections
Antimicrobial peptides
Seek & Heal™ targeting
Targeting verification
Antimicrobial substance
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Seek & Heal™ Particles: No Targeting of Blood







A recently infected wound
contains a mixture of blood
components, healthy tis-
sue and bacteria.

A product for wound disin-fection should selectively
kill the bacteria, while not
interfering with the other components in the wound liquid.

The diagram at right illu-
strate survival of Erythro-
cytes, i.e. red blood cells,
when exposed to AMS, an
antiseptic substance, and
AMS encapsulated in two
separated batches of
Seek & Heal™ particles.

Concentration of AMS is
indicated within brackets.

The vertical axis represents percent of cells not harmed.
  The horisontal axis represent time, up to about two hours.

No damage to Erythrocytes

The diagram above illustrates that AMS, i.e. the antimicrobial substance administered without Seek & Heal™
technology, gave a rather short, concentration dependent Erythrocyte survival time.

A formulation from the first generation of carriers gave an Erythrocyte survival time of about 12 minutes(E76).
Subsequent refinements of the Seek&Heal™ particles gave Erythrocyte survival times of about 25 SH29)
and 100 minutes (SH59).



Counting of Erythrocytes

Human blood was diluted in isotonic NaCl solution. At time =0, the diluted
blood was mixed with the solution to be studied. The mixture was added to
a hemocytometer, which was inserted into a microscope.


The sample was photographed at regular time intervals
(normally each 20 seconds). The Erythrocytes were then
counted from the images using an image analysis system.

The images at left and below show decline of erythrocytes
in one of the experiments.